immunological
Genetic Monitoring Program
Genetic monitoring is a system for quality control of breeding and production of laboratory animal. NALC-MU has established genetic monitoring program that focused on the screening of genetic integrity in inbred strains and heterogeneity maintained in outbred stock. The program is composed by observation and analysis of phenotypic expressions such as coat color, breeding performance, protein variant and cell surface antigen.
The routine genetic monitoring items to confirm the accuracy of the animal strain are performed by electrophoresis* and immunological method. The genetic marker items are presented in table 1**, 2 and 3. According to the program monitoring is presented in table 4.
| *Electrophoresis method that be used for allelic pattern checking of biochemical markers are : |
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· Cellulose acetate membrane electrophoresis (CAME) |
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· Polyacrylamide gel electrophoresis (PAGE) |
| **Table 1 List of Biochemical marker Items for Mice | ||||
| Locus | Chr.No. | Gene Name | Method | Sample |
| Idh1 | 1 | Isocitrate dehydrogenase-1 | CAME | Kidney |
| Pep3 | 1 | Peptidase-3 | CAME | Kidney |
| Akp1 | 1 | Alkaline phosphatase-1 | CAME | Kidney |
| Car2 | 3 | Carbonic anhydrase-2 | CAME | Hemolysate |
| Mup1 | 4 | Major urinary protein-1 | PAGE | Urine |
| Gpd1 | 4 | Glucose phosphate dehy | CAME | Kidney |
| Pgm1 | 5 | Phosphoglucomutase-1 | CAME | Kidney |
| Ldr1 | 6 | Lactate dehydrogenase regulator-1 | CAME | Hemolysate |
| Gpi1 | 7 | Glucose phosphate isomerase-1 | CAME | Hemolysate |
| Hbb | 7 | Hemoglobin beta chain | CAME | Hemolysate |
| Es1 | 8 | Esterase-1 | CAME | Plasma |
| Es1 | 8 | Esterase-2 | CAME | Kidney |
| Mod1 | 9 | Malic enzyme supernatant | CAME | Kidney |
| Trf | 9 | Transferrin | CAME | Plasma |
| Es3 | 11 | Esterase-3 | CAME | Kidney |
| Table 2 List of Biochemical marker Items for Rat | ||||
| Locus | Chr.No. | Gene Name | Method | Sample |
| Hbb | 1 | Hemoglobin beta chain | CAME | Hemolysate |
| Amy1 | 2 | Amylase-1 | CAME | Pancreas |
| Svp1 | 3 | Seminal vesicle protein-1 | CAME | Seminal |
| Acon1 | 5 | Aconitase-1 | CAME | Kidney |
| Mup1 | 5 | Major urinary protein-1 | PAGE | Urine |
| Fh1 | 13 | Fumarate hydratase-1 | CAME | Kidney |
| Gc | 14 | Group-specific component | CAME | Plasma |
| Es1 | 19 | Esterase-1 | PAGE | Plasma |
| Es2 | 19 | Esterase-2 | PAGE | Plasma |
| Es3 | 11 | Esterase-3 | PAGE | Intestine |
| Es4 | 19 | Esterase-4 | CAME | Kidney |
| Es14 | 19 | Esterase-14 | PAGE | Plasma |
