Environmental Monitoring system
Husbandry environment and equipment from Maximum Barrier and Low Barrier / Strict hygienic conventional (SH conventional) are regularly monitored to prevent contamination.
The health monitoring system is divided into two categories :
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Environmental monitoring system is divided into three categories
Chart of health monitoring system
-
Physical monitoring
- Food (Shape, Size, Color, Smell, Insect and Others)
- Water (Color, Smell and Turbidity)
- Bedding (Shape, Size, Smell, Sharp, Insect and Others)
- Air (Air change, Temperature, Humidity, and Pressure)
- Light intensity
-
Chemical monitoring
- Water (Chlorine and Vitamin C)
- Air (NH3, CO2, and O2)
- Food (Nutrition values)
-
Microbiological monitoring
- Food (Aerobic plate count (APC), Total coliform count (TCC), Salmonella and Sterility test)
- Water (APC, Pseudomonas aeruginosa detection and Sterility test)
- Bedding (APC, and Sterility test)
- Cage, transfer box (APC, and Sterility test)
- Air (APC)
- Wall, Floor (APC)
- Disinfectant (% Effective to reference bacteria)
Technique and Standard Criteria for Environmental monitoring
Item |
Criteria |
Technique/Equipment |
|
Maximum Barrier |
Low Barrier/ SH conventional |
||
Food |
Sterile |
APC* < 5000 cfu/g |
Mesophilic aerobic plate count |
Water |
- Sterile(autoclaved |
APC < 200 cfu/100ml |
Membrane filter technique |
Bedding |
Sterile |
Sterile |
Steam sterilization indicator tape |
Cage |
Sterile |
Sterile - APC < 10 cfu |
Steam sterilization tape |
Air in animal room |
APC 50 -100 cfu/m3 |
APC < 15 cfu/ft2/min |
Settle plate technique / Air sampler |
Air in clean storage room |
APC 50 -100 cfu/m3 |
APC < 15 cfu/ft2/min |
Settle plate technique / Air sampler |
Air in supporting area |
APC 50 -100 cfu/m3 |
APC < 15 cfu/ft2/min |
Settle plate technique / Air sampler |
Wall |
APC < 5 cfu |
ND |
RODAC Plate |
Floor |
APC < 15 cfu |
ND |
RODAC Plate |
Disinfectant control |
100 % Effective |
100% Effective |
Effective to standard bacteria |
Water for Sanitations |
20-50 ppm |
20-50 ppm |
Iodometric II |
Air change |
10 – 15 ACH |
10 – 15 ACH |
Anemometer |
Temperature |
18 - 25 oC |
18 - 25 oC |
Anemometer |
Humidity |
30 – 70 % |
30 – 70 % |
Anemometer |
Pressure |
positive |
positive |
HVAC/ |
Sound |
<85 dB |
<85 dB |
RION Integrating Sound Level Meter |
Light intensity |
150 -300 Lux |
150 -300 Lux |
Digital LUX Meter |
NH3 |
<25 ppm |
<25 ppm |
Gas Detector |
* ISO/IEC 17025
Environmental monitoring report 2009
Environment |
(No. of positive / No. of tested samples) |
|
---|---|---|
Test (Criteria) |
2009 |
|
Food No.082 · Autoclaved & radiation food |
· Sterility test (-ve) |
0/86 |
Food No.086 · Pasteurized food |
· APC < 5,000 cfu./g |
0/60 |
Food No.084 · Pasteurized food |
· APC < 106 cfu./g |
0/15 |
Water · Chlorinated water 10-12 ppm. |
· APC < 200 cfu./100 ml |
0/300 |
Water · Chlorinated water 3.5-4.0 ppm(Control) |
· APC < 200 cfu./100 ml |
0/24 |
Water · Chlorinated water 3.5-4.0 ppm. plus
|
· APC < 200 cfu./100 ml |
0/153 |
· Autoclaved water (Maximum barrier) |
· Sterility test (-ve) |
0/30 |
Cage · Maximum barrier |
· Sterility test (-ve) |
0/344 |
Air |
|
|
Wall |
· RODAC plate < 5 cfu./plate |
1/275 |
Bedding |
|
|
Others instruments in Maximum barrier |
· Sterility test (-ve) |
0/135 |