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Environmental Monitoring system

Written by Super User. Posted in EnvironmentMonitoring

          Husbandry environment and equipment from Maximum Barrier and Low Barrier / Strict hygienic conventional (SH conventional) are regularly monitored to prevent contamination.

 The health monitoring system is divided into two categories :
 


Environmental monitoring system is divided into three categories 
Chart of health monitoring system

  • Physical monitoring
         - Food (Shape, Size, Color, Smell, Insect and Others)
         - Water (Color, Smell and Turbidity)
         - Bedding (Shape, Size, Smell, Sharp, Insect and Others)
         - Air (Air change, Temperature, Humidity, and Pressure)
         - Light intensity
     
  • Chemical monitoring
         - Water (Chlorine and Vitamin C)
         - Air (NH3, CO2, and O2)
         - Food (Nutrition values)
     
  • Microbiological monitoring
         - Food (Aerobic plate count (APC), Total coliform count (TCC), Salmonella and Sterility test)
         - Water (APC, Pseudomonas aeruginosa detection and Sterility test)
         - Bedding (APC, and Sterility test)
         - Cage, transfer box (APC, and Sterility test)
         - Air (APC)
         - Wall, Floor (APC)
         - Disinfectant (% Effective to reference bacteria)

 

Technique and Standard Criteria for Environmental monitoring

Item

 Criteria

Technique/Equipment

Maximum Barrier

Low Barrier/ SH conventional

Food   

Sterile

APC*   <  5000 cfu/g
TCC*  <  3 MPN/g 
Salmonella*  = 0

Mesophilic aerobic plate count
Enumeration of coliform 
Detection of Salmonella

Water

- Sterile(autoclaved
   water) for IVC
- Chlorinated water
   10-12 ppm.

APC < 200 cfu/100ml
Pseudomonas aeruginosa = 0
Chlorinated water 10-12 ppm
Chlorinated water 3.5-4 ppm
Vitamin C 300-500 mg/l

Membrane filter technique
Detection of Pseudomonas aeruginosa
DPD (Cl2  analysis) / Colorimeter
DPD (Cl2  analysis) Colorimeter
Titration method (Iodine drop)

Bedding

Sterile

Sterile

Steam sterilization indicator tape
Steam biological indicator
Sterility test (Cultivation method)

Cage

Sterile

Sterile - APC < 10 cfu

Steam sterilization tape
Surface swab & Spread plate 
RODAC Plate

Air in animal room

APC 50 -100  cfu/m3

APC < 15 cfu/ft2/min

Settle plate technique / Air sampler

Air in clean storage room

APC 50 -100  cfu/m3

APC < 15 cfu/ft2/min

Settle plate technique / Air sampler

Air  in supporting area

APC 50 -100  cfu/m3

APC < 15  cfu/ft2/min

Settle plate technique / Air sampler

Wall

APC < 5  cfu

ND

RODAC Plate

Floor

APC < 15  cfu

ND

RODAC Plate

Disinfectant control

100 % Effective

100% Effective

Effective to standard bacteria   
106 cfu S. aureus           ATCC 6538 
106 cfu E. coli                ATCC 25922 
106 cfu P. aeruginosa    ATCC 27853 

Water  for Sanitations

20-50 ppm

20-50 ppm

 Iodometric II                                    
 DPD (Cl2 analysis) / Colorimeter

Air change

10 – 15 ACH

10 – 15 ACH

 Anemometer

Temperature

18 - 25  oC

18 - 25  oC

 Anemometer

Humidity

30 – 70 %

30 – 70 %

 Anemometer

Pressure

positive

positive

 HVAC/

Sound

<85 dB

<85 dB

 RION Integrating Sound Level Meter

Light intensity

150 -300 Lux

150 -300 Lux

 Digital LUX Meter

NH3 
CO2
O2

<25 ppm
<2,000 ppm
18-20%

 <25 ppm
<2,000 ppm
18-20%

 Gas Detector

* ISO/IEC 17025

Environmental monitoring report 2009

Environment

(No. of positive / No. of tested samples)

Test (Criteria)

2009

Food No.082  · Autoclaved & radiation  food
(Mouse & Rat) · Pasteurized food

·   Sterility test (-ve)
·   APC < 5,000 cfu./g
·   TCC < 3 MPN/g
·   Salmonella  =  not detected / 25 g

0/86
0/120
0/120
0/120

Food No.086 · Pasteurized food
(Guinea pig & Rabbit)

·   APC < 5,000 cfu./g
·   TCC < 3 MPN/g
·   Salmonella  =  not detected / 25 g

0/60
0/60
0/60

Food No.084 · Pasteurized food 
(Sheep)

·   APC < 106 cfu./g
·   Salmonella   =  not detected / 25 g

0/15
0/15

Water   · Chlorinated water 10-12 ppm.
(Mouse, Rat & Rabbit ) 

·   APC < 200 cfu./100 ml
·   Pseudomonas aeruginosa = not 
    detected / 100 ml.
·   Total chlorine = 10-12 ppm.
·   Free chlorine = 10-12 ppm.

0/300
0/300

7/73
7/99

Water   · Chlorinated water 3.5-4.0 ppm(Control)     

·   APC < 200 cfu./100 ml
·   Pseudomonas aeruginosa = not 
     detected/100 ml.
·   Total chlorine = 3.5-4.0 ppm.
·    Free chlorine = 3.5-4.0 ppm.

0/24
0/24

6/23
5/23

Water   ·  Chlorinated water 3.5-4.0 ppm. plus
  (Guinea pig)    Vitamin C 500 mg/l

              

·   APC < 200 cfu./100 ml
·   Pseudomonas aeruginosa = not
     detected /100 ml.
·   Vitamin C = 300-500 mg/l

0/153
0/153

6/153

            · Autoclaved water (Maximum barrier)

 ·   Sterility test  (-ve)

0/30

Cage  ·  Maximum  barrier
            ·  Low barrier

·   Sterility test  (-ve)
·   APC < 10 cfu/plate

0/344
27/278

Air      
           ·    Low barrier
           ·    Maximum barrier

           ·   Supporting  area
           ·   Clean storage room


·   Settle plate < 15 cfu./ ft2 / min
·   Settle plate < 5 cfu./ ft2 / min
·   Air sampler  50-100 cfu./m3
·   Settle plate < 15 cfu./ ft2/ min
·   Settle plate < 5 cfu./ ft2 / min

 
11/168
0/173
0/65
15/255
27/245

Wall
Floor

·   RODAC plate < 5 cfu./plate
·   RODAC plate < 15 cfu./plate

1/275
0/109

Bedding
· Wood shaving (for Maximum barrier,
   Low barrier,and Strict hygienic conventional)
· Corn cob (for Low barrier)


·   Sterility test  (-ve)
·   APC = 0 cfu/g
·   Biological steam sterilization
    Indicator = -ve


0/77
0/46
1/114

Others instruments in Maximum barrier
Animal transportation box

·   Sterility test  (-ve)
·   APC < 15 cfu/plate

0/135
19/160